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We observed that zinc supplementation to healthy human subjects aged 20–50 years decreased the concentration of MDA (malondialdehyde), 4 hydroxy alkenals (HAE), and 8-hydroxy deoxyguanine in the plasma ( ex vivo induction of TNF-α and IL-1β mRNA in mononuclear cells were inhibited in zinc supplemented subjects and this resulted in decreased TNF-α induced nuclear factor-κB (NF-κB) activation in isolated MNCs (mononuclear cells) ( In order to understand the mechanism of zinc effect on cell-mediated immunity, we utilized RT-PCR analysis to determine PHA (phytohemagglutinins) induced expression of IL-2 mRNA in isolated MNCs in elderly subjects before and after zinc supplementation

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The aging field now faces a critical methodological question in translating biological insights into actionable clinical results: how best to measure the effects of an intervention on biological aging from data collected in clinical trials